王芳（Wang fang）：

性别： 女

职称：教授（硕士、博士生导师）

通讯地址：

武汉大学药学院 药物科学系

武汉市武昌区东湖路185号 邮编：430071 

学习经历：

2002.09 — 2007.07 武汉大学化学与分子科学学院，理学博士

2006.04 — 2007.01 香港浸会大学，理学院化学系，联合研究

1998.09 — 2002.07 武汉大学化学与分子科学学院，国家化学人才培养基地班，理学学士

2001.09 — 2002.06 中国科学院，北京化学所，联合培养 

工作经历：

2022.12 —至今 武汉大学药学院 教授

2009.12 — 2022.11 武汉大学药学院 副教授

2014.08 — 2016-11   美国芝加哥大学 博士后 导师：何川教授

2007.07 — 2009.11   武汉大学药学院 讲师 

获奖情况：

2017-2019 武汉大学珞珈青年学者

2019 第六届全国医药院校药学中药学专业大学生实验技能竞赛项目负责人 三等奖两人次

2019全国高等学校药学类专业青年教师微课大赛 一等奖

2019全国高等学校药学类专业青年教师教学能力大赛 二等奖

2010年湖北省科学技术奖励自然科学奖二等奖（第5完成人） 

研究兴趣与方向：

1. 电化学纳米生物传感器的制备与应用；

2. 核酸甲基化的检测及相关蛋白酶的活性分析；

3. 药物与生物大分子相互作用研究；

4. 药物色谱分离与电化学检测联用。 

承担课题：

国家自然科学基金面上项目 22277094 主持

国家自然科学基金面上项目 21778041 主持

国家自然科学基金青年科学基金项目 60801020主持

湖北省自然科学基金面上项目  ZRY2014000492主持

中央高校基本科研业务费专项资金2042014kf0295主持

武汉大学珞珈青年学者人才基金 主持

湖北省教育厅教改项目 主持

武大大学教改项目 主持

武大大学医学部教改项目 主持

国家科技部973计划 2012CB720600 参与（骨干）

国家自然科学基金重大研究计划重点支持项目91753201 参与（骨干）

国家自然科学基金重大研究计划重点项目  90817103 参与（骨干）

代表性论文：

1. Ying Cheng^#, Chen Chen^#, Fang Wang*, Zilin Chen*. A highly sensitive signal-on biosensor based on restriction enzyme-mediated molecular switch for detection of TET1. Bioelectrochemistry, 2023, 152: 108433.

2. Mei Zhao^#, Guangrong Zou^#, Jing Tang, Jingyi Guo, Fang Wang*, Zilin Chen*. Probe-labeled electrochemical approach for highly selective detection of 5-carboxycytosine in DNA. Analytica Chimica Acta, 2023, 1273: 341521.

3. Hongmei Yang^#, Jing Ren^#, Mei Zhao, Chen Chen, Fang Wang*, Zilin Chen*. Novel electrochemical immunosensor for O6-methylguanine-DNA methyltransferase gene methylation based on graphene oxide-magnetic nanoparticles-β-cyclodextrin nanocomposite. Bioelectrochemistry, 2022, 146: 108111.

4. Tingting Zhang^#, Xiaoyan Liao^#, Jing Ren^#, Chen Chen, Yuying Wan, Fang Wang*, Zilin Chen*. Construction of ERGO film modified electrode and its combination with HPLC in determination of five active components in Osmanthus fragrans fruits. Chinese Journal of Analytical Chemistry, 2022, 50: 100109.

5. Jing Tang^#, Guangrong Zou^#, Chen Chen, Jing Ren, Fang Wang*, Zilin Chen*. Highly selective electrochemical detection of 5Formyluracil relying on (2-Benzimidazolyl) acetonitrile labeling. Analytical Chemistry, 2021, 93: 16439–16446 (journal’s cover).

6. Ying Cheng, Jing Tang, Xue Chen, Fang Wang*, Zilin Chen*. Assay for TET1 activity and its inhibitors screening with signal amplification by nanoparticles and Ru(III) redox recycling. Journal of Pharmaceutical and Biomedical Analysis. 2021, 203: 114228.

7. Xue Chen^#, Ying Cheng^#, Yafen Wang, Fang Wang*, Zilin Chen*. Fluorescence assay based on thioflavin T-induced conformation switch of G-quadruplex for TET1 detection. Analyst, 2021, 146: 2126-2130 (journal’s cover).

8. Zhenya Yu^#, Xue Chen^#, Ying Cheng, Hongmei Yang, Fang Wang*, Zilin Chen*. Novel label-free electrochemical strategy for sensitive determination of ten-eleven translocation protein 1. Analytica Chimica Acta, 2021, 1146: 140-145.

9. Hongmei Yang^#, Peiyu Hu^#, Jing Tang, Ying Cheng, Fang Wang*, Zilin Chen*. A bifunctional electrochemical aptasensor based on AuNPs-coated ERGO nanosheets for sensitive detection of adenosine and thrombin. Journal of Solid State Electrochemistry, 2021, 25: 1383-1391.

10. Hongmei Yang^#, Yafen Wang^#, Jing Tang, Fang Wang*, Zilin Chen*. End-labeling-based electrochemical strategy for detection of adenine methylation in nucleic acid. Microchimica Acta, 2021, 188: 250.

11. Wei Li, Hongmei Yang, Yafen Wang, Xiaocheng Weng*, Fang Wang*. Highly sensitive detection of 6mA at single-base resolution based on A–C mismatch. Analyst, 2021, 146: 4450-4453.

12. Xiaocheng Weng^#, Jing Gong^#,      Yi Chen^#, Tong Wu^#, Fang Wang, Shixi Yang, Yushu Yuan, Guanzheng Luo, Kai Chen, Lulu      Hu, Honghui Ma, Pingluan Wang, Qiangfeng Cliff Zhang*, Xiang Zhou*, Chuan      He*. Keth-seq for transcriptome-wide RNA structure mapping. Nature Chemical      Biology, 2020, 16: 489-492.

13. Wei Li, Weiwu Zeng, Yi Chen, Fang Wang, Fan Wu, Xiaocheng Weng*,      Xiang Zhou*. Biotinylation and isolation of an RNA G-quadruplex based on      its peroxidase-mimicking activity. Analyst, 2019, 144: 4472-4476.

14. Yi Chen, Shixi Yang, Shuang Peng, Wei Li, Fan      Wu, Qian Yao, Fang Wang, Xiaocheng      Weng*, Xiang Zhou*. N1-Methyladenosine detection with CRISPR-Cas13a/C2c2. Chemical Science, 2019, 10: 2975-2979.

15. Guanzheng Luo^#, Fang      Wang^#, Xiaocheng Weng, Kai Chen, Ziyang Hao, Miao Yu,      Xin Deng, Jianzhao Liu, Chuan He, Characterization of eukaryotic DNA      N6-methyladenine by a highly sensitive restriction enzyme-assisted      sequencing. Nature Communications, 2016, 7, 11301 (co-first author).

16. Lili Wang, Feiyue Yu, Fang Wang*, Zilin Chen*, Electrochemical detection of DNA methylation using a glassy carbon electrode modified with a composite made from carbon nanotubes and beta-cyclodextrin. Journal of Solid State Electrochemistry, 2016, 20(5): 1263-1270.

17. Lili Wang, Wencheng Gong, Fang Wang*, Zhenya Yu, Zilin Chen*, Efficient bienzyme nanocomposite film for chiral recognition of L-tryptophan, L-phenylalanine and L-tyrosine. Analytical Methods, 2016, 8(17): 3481-3487.

18. Fang Wang*, Wencheng Gong, Lili Wang, Zilin Chen*, Selective recognition of D-tryptophan from d/l-tryptophan mixtures in the presence of Cu(II) by electropolymerized L-lysine film. Analytical Biochemistry, 2016, 492: 30-33.

19. Fang Wang*, Fangfang Yan, Yuling Long, Lili Wang, Zilin Chen*, Interaction with Deoxyribonucleic Acid and Determination of Orientin in Lophatherum gracile Brongn by High-Performance Liquid Chromatography with Amperometric Detection. Electrochimica Acta, 2015, 178: 829-837.

20. Fang Wang*, Wencheng Gong, Lili Wang, Zilin Chen*, Enhanced amperometric response of a glucose oxidase and horseradish peroxidase based bienzyme glucose biosensor modified with a film of polymerized toluidine blue containing reduced graphene oxide. Microchimica Acta, 2015, 182(11-12): 1949-1956.

21. Fang Wang, Xianping Sun, Yan Chen, ShijingXie, Zilin Chen*, Non-Enzymatic Glucose Sensor Based on Copper Nanoparticles Modified Microelectrode. Nanoscience and Nanotechnology Letters, 2013, 5(6): 624-629.

22. Fangfang Yan, Fang Wang*, Zilin Chen*, Aptamer-based electrochemical biosensor for label-free voltammetric detection of thrombin and adenosine. Sensors and Actuators B-Chemical, 2011, 160(1): 1380-1385.

23. Fang Wang, Xiaocui Deng, Wei Wang, Zilin Chen*, Nitric oxide measurement in biological and pharmaceutical samples by an electrochemical sensor. Journal of Solid State Electrochemistry, 2011, 15(4): 829-836.

24. Fang Wang, Xiaowei Chen, Zilin Chen*, Eletrodeposited nickel oxide on a film of carbon nanotubes for monitoring nitric oxide release from rat kidney and drug samples. Microchimica Acta, 2011, 173(1-2): 65-72.

25. Fang Wang, Yaqian Liao, Yi Ren, Zilin Chen*, A novel method for D-arabinitol determination based on a nano-structured sensing film by one-step electrodeposition. Microchimica Acta, 2010, 170:9–15