赵昌明

性别：男

职务/职称: 教授

通讯地址（含邮编）：

湖北省武汉市武昌区东湖路185号武汉大学药学院，430071

办公地址：湖北省武汉市珞珈山武汉大学测试中心“组合生物合成与新药发现教育部重点实验室”C409

研究兴趣:

天然产物（Natural product）是开发小分子药物的宝贵资源库，临床使用的化学药物近一半来源于天然产物及其衍生物。本人的兴趣集中在抗感染、抗肿瘤、抗衰老等活性天然产物的生物合成、合成生物学及其作用机制研究。1）天然产物生物合成中的杂原子成键反应，包括碳-硫键、碳-磷键、碳-硒键形成的酶学机制；2）“长寿维生素”麦角硫因、卵硫醇、麦角硒因抗衰老的分子机制。

学习经历：

2017.9---2019.12，波士顿大学，化学系，访问学者

2010.6---2011.12，伊利诺伊大学香槟校区（UIUC）基因组生物研究所，博士后

2008.6---2010.5，上海交通大学，生命科学学院，博士后

2001.9---2007.12，华中农业大学，生命科学技术学院，博士

1997.9---2001.7， 华中农业大学，生命科学技术学院，本科

工作经历：

2020.12---现在，武汉大学药学院，教授

2012.1---2020.11，武汉大学药学院，副教授

主讲课程:

基因工程，生物技术制药

发表的研究论文（代表作）：

1. Chen L#, Zhang L#, Ye X*, Deng Z*, Zhao C*. Ergothioneine and its congeners: Anti-ageing mechanisms and pharmacophore biosynthesis. Protein Cell. 2023 Aug 10:pwad048. doi: 10.1093/procel/pwad048.

2. Chen L#, Zhang LS#, Ye C#, Zhou H, Liu B, Gao B, Deng Z, Zhao C*, He C*, Dickinson BC*.Nm-Mut-seq: a base-resolution quantitative method for mapping transcriptome-wide 2'-O-methylation. Cell Res. 2023 Sep;33(9):727-730. doi: 10.1038/s41422-023-00836-w.

3. Chen L, Deng Z, Zhao C*. Nitrogen-Nitrogen bond formation reactions involved in natural product biosynthesis. ACS Chem Biol. 2021 Apr 16;16(4):559-570. doi: 10.1021/acschembio.1c00052.

4. Deng Q, Liu Y, Chen L, Xu M, Naowarojna N, Lee N, Chen L, Zhu D, Hong X, Deng Z, Liu P*, Zhao C*. Biochemical characterization of a multifunctional mononuclear nonheme iron enzyme (PtlD) in neopentalenoketolactone biosynthesis. Org Lett. 2019 Sep 20;21(18):7592-7596. doi: 10.1021/acs.orglett.9b02872.

5. Deng Q, Xiao L, Liu Y, Zhang L, Deng Z, Zhao C*. Streptomyces avermitilis industrial strain as cell factory for Ivermectin B1a production. Synth Syst Biotechnol. 2019 Ma;4(1):34-39. doi: 10.1016/j.synbio.2018.12.003

6. Chen L#, Naowarojna N#, Chen B, Xu M,  Quill M, Wang J,  Deng Z,  Zhao C*, and  Liu P*. Mechanistic Studies of a Nonheme Iron Enzyme OvoA in Ovothiol Biosynthesis Using a Tyrosine Analogue, 2-Amino-3-(4-hydroxy-3-(methoxyl) phenyl) Propanoic Acid (MeOTyr). ACS Catal. 2019, 9(1): 253-258. doi: 10.1021/acscatal.8b03903

7. Naowarojna N#, Cheng R#, Chen L#, Quill M, Xu M, Zhao C*, Liu P*. Mini-Review: Ergothioneine and ovothiol biosyntheses, an unprecedented trans-sulfur strategy in natural product biosynthesis. Biochemistry. 2018 Jun 19;57(24):3309-3325. doi: 10.1021/acs.biochem.8b00239.

8. Chen L, Naowarojna N, Song H, Wang S, Wang J, Deng Z, Zhao C*, Liu P*. Use of a tyrosine analogue to modulate the two activities of a nonheme iron enzyme OvoA in ovothiol biosynthesis, cysteine oxidation versus oxidative C-S bond formation. J Am Chem Soc. 2018 Apr 4;140(13):4604-4612. doi: 10.1021/jacs.7b13628.

9. Deng Q, Zhou L, Luo M, Deng Z, Zhao C*. Heterologous expression of Avermectins biosynthetic gene cluster by construction of a Bacterial Artificial Chromosome library of the producers. Synth Syst Biotechnol. 2017 Mar 24;2(1):59-64. doi: 10.1016/j.synbio.2017.03.001.

10. Li M, Chen L, Deng Z, Zhao C*. Characterization of AmtA, an amidinotransferase involved in the biosynthesis of phaseolotoxins. FEBS Open Bio. 2016 May 16;6(6):603-609. doi: 10.1002/2211-5463.12071.

11. Chen L, Li P, Deng Z, Zhao C*. Ornithine transcarbamylase ArgK plays a dual role for the self-defense of phaseolotoxin producing Pseudomonas syringae pv.phaseolicola. Sci Rep. 2015 Aug 10;5:12892. doi: 10.1038/srep12892.

12. Hao C#, Huang S#, Deng Z, Zhao C*, Yu Y*. Mining of the pyrrolamide antibiotics analogs in Streptomyces netropsis reveals the amidohydrolase-dependent “iterative strategy” underlying the pyrrole polymerization. PLoS One. 2014 Jun 5;9(6):e99077. doi: 10.1371/journal.pone.0099077.

13. Evans BS*, Zhao C, Gao J, Evans CM, Ju KS, Doroghazi JR, van der Donk WA, Kelleher NL, Metcalf WW*. Discovery of the antibiotic phosacetamycin via a new mass spectrometry-based method for phosphonic acid detection. ACS Chem Biol. 2013 May 17;8(5):908-913. doi: 10.1021/cb400102t.

14. Zhao C, Huang T, Chen W, Deng Z*. Enhancement of the diversity of polyoxins by a thymine-7-hydroxylase homolog outside the polyoxin biosynthesis gene cluster. Appl Environ Microbiol. 2010. 76(21):7343-7347

15. Zhao C, Song C, Yu Z and Sun M*. L-2,3-diaminopropionate: one of the building blocks for the biosynthesis of Zwittermicin A in Bacillus thuringiensis subsp. kurstaki strain YBT-1520. FEBS Lett. 2008. 582(20):3125-3131

16. Zhao C, Zeng H, Yu Z and Sun M*. N-acyl homoserine lactonase promotes prevention of Erwinia virulence with Zwittermicin A producing strain Bacillus cereus. Biotechnol Bioeng. 2008. 100(3):599-603

17. Zhao C, Luo Y, Song C, Liu Z, Chen S, Yu Z and Sun M*. Identification of three Zwittermicin A biosynthesis-related genes from Bacillus thuringiensis subsp. kurstaki strain YBT-1520. Arch Microbiol. 2007. 187(4):313-319

参编教材：

《基因工程》第二版，孙明主编，高等教育出版社，2013.

《基因工程》第三版，孙明主编，高等教育出版社，2023.