龙凤
性别：女
职务/职称：教授
通讯地址：
武汉大学测试中心C505室，430070
学习简历：
1996.09 - 2000.07 华中农业大学，学士（本硕连读）
1999.09 - 2002.07 华中农业大学，硕士
2004.08 - 2010.08 美国爱荷华州立大学，博士
工作简历：
2002.09 – 2004.08 上海睿星基因技术有限公司，科研助理
2010.08 - 2012.11 美国爱荷华州立大学，博士后
2012.12 - 2017.03 美国普渡大学，博士后
2017.04 - 2018.10 美国普渡大学，助理研究科学家
2018.11-2020.11 武汉大学，药学院，特聘研究员
2020.12-至今 武汉大学，药学院，教授
研究领域:
生物大分子的结构和功能研究
研究兴趣与方向:
本课题组的研究兴趣在于，通过揭示重要生物大分子及其复合物的结构和功能，破译重要流行性病原微生物和人类重大疾病的相关病理发生机制，为生物防治的完善和进步提供理论依据和发展方向。课题研究主要涉及，结合应用冷冻电镜、X-射线晶体学、细胞生物学、分子生物学和生物化学等多个交叉学科的技术和研究方法，以解析生物大分子的三维结构为核心基础，对生物分子的作用机制和细胞机理进行深入探讨和阐述。
主要研究方向包括：
1. 流行性病毒的宿主侵染机制
2. 病毒的细胞免疫逃逸机制
3. 重要疾病的药物靶点挖掘和药物作用机制
4. 细胞的耐药性机制
代表性论文:
1. Huang, W., Y. He, R. Jiang, Z. Deng, F. Long*. (2022) Functional and Structural Dissection of a Plant Steroid 3-O-Glycosyltransferase Facilitated Engineering Enhancement of Sugar Donor Promiscuity. ACS Catalysis. 12(5):2927-2937
2. Sevvana, M., T. F. Rogers, A. S. Miller, F. Long, T. Klose, N. Beutler, Y. C. Lai, M. Parren, L. M. Walker, G. Buda, D. R. Burton, M. G. Rossmann, R. J. Kuhn. (2020) Structural basis of zika virus specific neutralization in subsequent flavivirus infections. Viruses. 12(12):1346
3. Long, F., M. Doyle, E. Fernandez, A. S. Miller, T. Klose, M. Sevvana, A. Bryan, E. Davidson, B. J. Doranz, R. J. Kuhn, M. S. Diamond, J. E. Crowe, M. G. Rossmann. (2019) Structural basis of a potent human monoclonal antibody against Zika virus targeting a quaternary epitope. Proc. Natl. Acad. Sci. USA. 116 (5):1591-1596
4. Sevvana, M., F. Long, A. Miller, T. Klose, G. Buda, L. Sun, R. J. Kuhn, and M. G. Rossmann. (2018) Refinement and analysis of the mature zika virus Cryo-EM structure at 3.1 resolution. Structure. 26(9):1169-1177.e3
5. Hasan, S. S., A. Miller, G. Sapparapu, T. Klose, F. Long, E. Fernandez, J. C. Porta, W. Jiang, M. S. Diamond, J. E. Crowe, Jr., R. J. Kuhn, M. G. Rossmann. (2016) A potently neutralizing human antibody cross-links Zika virus to prevent infection. Nat. Commun. 8:14722
6. Long, F., R. H. Fong, S. K. Austin, Z. Chen, T. Klose, A. Fokine, Y. Liu, J. Porta, G. Sapparapu, W. Akahata, B. J. Doranz, J. E. Crowe, M. S. Diamond, M. G. Rossmann. (2015) Cryo-EM structures elucidate neutralizing mechanisms of anti-chikungunya human monoclonal antibodies with therapeutic activity. Proc. Natl. Acad. Sci. USA 112(45):13898-13903
7. Fox, J. M., F. Long, M. A. Edeling, H. Lin, M. Duijl-Richter, R. H. Fong, K. M. Kahle, J. M. Smith, J. Jin, G. Simmons, B. J. Doranz, J. E. Crowe, D. H. Fremont, M. G. Rossmann, M. S. Diamond. (2015) Broadly neutralizing alphavirus antibodies bind an epitope on E2 and inhibit entry and egress. Cell 163(5):1095-1107
8. Su, C.-C.#, Long, F.#, M. T. Zimmermann, R. L. Jernigan, and E. W. Yu. (2011) Crystal structure of the CusBA heavy-metal efflux complex of Escherichia coli. Nature 470:558-562. (#共同第一作者)
9. Long, F.#, Su, C.-C.#, M. T. Zimmermann, S. E. Boyken, K. R. Rajashankar, R. L. Jernigan, and E. W. Yu. (2010) Crystal structures of the CusA efflux pump suggest methionine-mediated metal transport. Nature 467:484-488.（#共同第一作者）
10. Liu, Y., J. Sheng, A. Fokine, G. Meng, W. H. Shin, F. Long, R. J. Kuhn, D. Kihara, M. G. Rossmann. (2015) Structure and inhibition of EV-D68, a virus that causes respiratory illness in children. Science 347(6217):71-74.
11. Long, F., C.-C. Su, H.-T. Lei, J. Bolla, S. V. Do, and E. W. Yu. (2012) Structure and mechanism of the tripartite CusCBA heavy-metal efflux complex. Philosophical Transactions B 367:1047-1058.
12. Su, C.-C. #, Long, F. #, H.-T. Lei, J. Bolla, S. V. Do, K. R. Rajashankar and E. W. Yu. (2012) Charged amino acids (R83, E567, D617, E625, R669 and K678) of CusA are required for metal-ion transport in the Cus efflux system. J. Mol. Biol. 422:429-441. (#共同第一作者)
13. Bolla, J. R., S. V. Do, F. Long, L. Dai, C.-C. Su, H.-T. Lei, X. Chen, J. E. Gerkey, D. C. Murphy, K. R. Rajashankar, and E. W. Yu. (2012) Structural and functional analysis of the transcriptional regulator Rv3066 of Mycobacterium tuberculosis. Nucl. Acids Res. 40(18):9340-9355
14. Bolla, J. R., Su, C.-C., J. A. Delmar, A. Radhakrishnan, N. Kumar, T.H. Chou, F. Long, K. R. Rajashankar, E. W. Yu. (2015) Crystal structure of the Alcanivorax borkumensis YdaH transporter reveals an unusual topology. Nat. Commun. 6:6874
15. Su, C.-C., Radhakrishnan, A., Kumar, N., F. Long, J. R. Bolla, H.-T. Lei, J. A. Delmar, S. V. Do, T.-H. Chou, K. R. Rajashankar, Q Zhang, and E. W. Yu. (2014) Crystal structure of the Campylobacter jejuni CmeC outer membrane channel. Prot. Sci. 23: 954–961.
16. Long, F., C. Rouquette-Loughlin, W. M. Shafer, and E. W. Yu. (2008) Functional cloning and characterization of the multidrug efflux pumps NorM from Neisseria gonorrhoeae and YdhE from Escherichia coli. Antimicrob. Agents Chemother. 52(9):3052-60.
17. Su, C.-C., Bolla, J. R., N. Kumar, A. Radhakrishnan, F. Long, J. A. Delmar, T.-H. Chou, K. R. Rajashankar, W. M. Shafer, E. W. Yu. (2015) Structure and function of Neisseria gonorrhoeae MtrF illuminates a class of antimetabolite efflux pumps. Cell Rep. 11(1):61-70
18. Lei, H-T, Chou, T-H, Su, C-C, J. R. Bolla, N. Kumar, A. Radhakrishnan, F. Long, J. A. Delmar, S. V. Do, K. R. Rajashankar, W. M. Shafer, E. W. Yu. (2014) Crystal structure of the open state of the Neisseria gonorrhoeae MtrE outer membrane channel. PLoS ONE 9(6): e97475.
19. Bolla, J. R., Su, C.-C., Do, S. V., A. Radhakrishnan, N. Kumar, F. Long, T.-H. Chou, J. A. Delmar, H.-T. Lei, K. R. Rajashankar, W. M. Shafer, E. W. Yu. (2014) Crystal structure of the Neisseria gonorrhoeae MtrD inner membrane multidrug efflux pump. PLoS ONE 9(6): e97903.
20. Su, C.-C., F. Long, and E. W. Yu. (2010) The Cus system removes toxic metal ions via a methionine shuttle. Prot. Sci. 20:6-18. (同期杂志封面)
21. Su, C.-C., F. Yang, F. Long, D. Reyon, M. D. Routh, D. W. Kuo, A. K. Mokhtari, J. D. Van Ornam, K. L. Rabe, J. A. Hoy, Y. J. Lee, K. R. Rajashankar, and E. W. Yu. (2009) Crystal structure of the membrane fusion protein CusB from Escherichia coli. J. Mol. Biol. 393(2):342-55.
22. Su, C.-C., F. Long, G. McDermott, W. M. Shafer, and E. W. Yu. (2008) Crystallization and preliminary X-ray diffraction analysis of the multidrug efflux transporter NorM from Neisseria gonorrhoeae. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64:289-92.
23. Gu, R., M. Li, C.-C. Su, F. Long, M .D. Routh, F. Yang, G. McDermott, and E. W. Yu. (2008) Conformational change of the AcrR regulator reveals a possible mechanism of induction. Acta Crystallogr Sect. F Struct. Biol. Cryst. Commun. 64:584-8.
24. Li, M., X. Qiu, C.-C. Su, F. Long, R. Gu, G. McDermott, and E. W. Yu. (2006) Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the regulator AcrR from Escherichia coli. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62:1150-2.
25. Takano, E., Tao, M., F. Long, M. J. Bibb, L. Wang, W. Li, M. J. Buttner, M. J. Bibb, Z. X. Deng, and K. F. Chater. (2003) A rare leucine codon in adpA is implicated in the morphological defect of bldA mutants of Streptomyces coelicolor. Mol. Microbiol. 50(2):475-86.)
著作:
1. Do, S. V., C.-C. Su, F. Long, H.-T. Lei, J. R. Bolla and E. W. Yu. (2013) Structure, mechanism and assembly of the tripartite CusCBA heavy metal efflux complex. Microbial Efflux Pumps: Current Research :51. (Book Chapter)
2. Su, C.-C., F. Long, and E. W. Yu. (2012) CusBA heavy metal efflux pump of E. coli. Encyclopedia of Inorganic and Bioinorganic Chemistry DOI: 10.1002/9781119951438.eibc2054. (Book Chapter)
3. Routh, M. D., Y. Zalucki, C.-C. Su, F. Long, Q. Zhang, W. M. Shafer, and E. W. Yu. (2011) Efflux pumps of the resistance-nodulation-division family: a perspective of their structure, function, and regulation in Gram-negative bacteria. Advances in Enzymology and Related Areas of Molecular Biology 77:109-146. (Book Chapter)
招生招聘：
因课题研究需要，现招聘1-2名博士后研究人员（35岁以下）；具有蛋白、生化、细胞等相关研究背景，结构生物学背景优先；具有较好的英语阅读和写作能力，在主流SCI期刊上以（共同）第一作者发表过研究论文；工作积极，认真负责，独立工作能力强，团队意识强；具体薪酬根据相关学校政策以及个人教育经历和研究背景有所浮动，年收入约为18-30万元（税前）；可申请入住学校博士后公寓；课题组支持和推荐申请博士后科学基金及重点博士后支持项目。应聘者请将个人详细简历及推荐人（1-2位）联系方式等相关材料发送至：longfe@whu.edu.cn。邮件标题请注明：应聘博士后+姓名。初选通过后，邮件通知候选人。
课题组长期欢迎本科生加入实验室实习，进行大创或毕设的研究；欢迎对本实验室研究方向感兴趣的有志青年踊跃报考硕士和博士。
